Fig. 3.

Correlation between the number of nuclei labeled for DNA in situ and the amount of extractable nuclear DNA in hair. Hairs of different individuals were stained for DNA, and nuclei were counted under the microscope. A separate batch (5 mg) of hairs of the same individual was then used for DNA extraction and quantification of nuclear and mitochondrial DNA by real-time PCR. Spearman’s rank correlation coefficient (rho) and the significance of correlation were determined for the frequency of DNA-positive nuclei and nuclear DNA (a), the frequency of DNA-positive nuclei and mitochondrial DNA (b), and the amounts of nuclear DNA and mitochondrial DNA (c). rho, Spearman’s rank correlation coefficient; n.s., not significant