Figure 6.

OPA1 mutation increases Bax expression, but decreases Bcl-xL and pBad expression in Opa1^enu/+ mice. (a) Opa1^enu/+ mice significantly increased Bax mRNA and protein expression compared with wild-type control mice. (b) Opa1^enu/+ mice significantly increased Bax protein expression, but decreased Bcl-xL and pBad protein expression compared with wild-type control mice. Values are mean±S.D. (n=4 retinas/group). ^*Significant at P<0.05 compared with wild-type control mice. (c–e) Bcl-xL immunohistochemistry (green). When the primary antibody for Bcl-xL was omitted, there was no binding of the secondary antibody (c). Compared wild-type control mouse (d), Opa1^enu/+ mouse showed decreased Bcl-xL immunoreactivity in the IPL and GCL (e). ONL, outer nuclear layer; OPL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar, 20 μm (c–e)