Figure 5.
Effect of c-FLIP isoform knockdown on the susceptibility of urothelial carcinoma cells to TRAIL-induced apoptosis. (a and b) VMCub1 (a) or SD cells (b) with knockdown of c-FLIPL, c-FLIPS or both isoforms (c-FLIPL/S) were left untreated or stimulated with 25 ng/ml TRAIL for 24 h. The amount of apoptotic cells was quantified by measuring the sub-G1 DNA content by flow cytometry. Data are shown as the mean of at least three measurements±S.D. (c and d) For analysis of caspase-8 and caspase-3 cleavage, VMCub1 (c) and SD cells (d) were left untreated or stimulated with 25 ng/ml TRAIL for the times as indicated. Lysates were analysed by western blot with β-actin as the loading control