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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Biochem Pharmacol. 2011 Jul 23;82(9):1100–1109. doi: 10.1016/j.bcp.2011.07.078

Figure 3. Effect of VCN-2 on apoptotic, proliferative and tumor suppressor proteins in CaP.

Figure 3

Androgen-dependent (LNCaP) and androgen-independent (PC3) control and 50 μM VCN-2 treated cells were processed for Western-blot for PARP-cleavage and apoptosis regulatory proteins Bcl2 and Bax along with fibronectin and E-cadherin using specific antibodies (panel A). Western-blot analysis of the activation of pEGFR (Y1068), pAkt (S473) and pP70S6K (S240-244) in LNCaP and PC3 cells following VCN-2 treatment (panel B). Western-blot for the levels of cell-cycle regulatory proteins cyclin B1, cyclin D1, CDK4, PCNA and hTERT (panel C). Western-blot for oncoprotein C-Myc, and tumor suppressors Rb, pRb (S780) p53 and p21 (panel D). Membranes were stripped and reprobed for GAPDH as a loading control.