TABLE 1 .
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or reference(s) |
|---|---|---|
| Strain | ||
| MJM1100 | Sequenced wild-type V. fischeri ES114 | 30, 43 |
| TIM305 | ES114 ΔluxO | 11 |
| TIM355 | ES114 ΔluxO ΔlitR | 11 |
| TIM358 | ES114 ΔlitR | 11 |
| TIM313 | ES114 Tn7::pEVS107 (erm) | 11 |
| TIM315 | ES114 ΔluxO Tn7::pEVS107 (erm) | 11 |
| TIM366 | ES114 Tn7::pTM319 (Ptrc-rpoQ) | This study |
| CA1 | ES114 ΔrpoQ | This study |
| CA4 | ES114 ΔluxO ΔrpoQ | This study |
| CA6 | ES114 ΔluxO ΔrpoQ Tn7::rpoQ | This study |
| CA8 | ES114 ΔVFA1014 | This study |
| CA19 | ES114 ΔlitR Tn7::pEVS107 (erm) | This study |
| KV1612 | ES114 VFA1017::kan | 66 |
| AMJ2 | ES114 ΔarcA | 38 |
| Plasmid | ||
| pEVS79 | pBC SK(+) oriT cat | 62 |
| pEVS104 | R6KoriRP4 oriT trb tra kan | 62 |
| pEVS107 | R6Kori oriT mini-Tn7 mob erm kan | 64 |
| pVSV105 | R6Kori ori(pES213) RP4 oriT cat | 67 |
| pUX-BF13 | R6Kori tns bla | 65 |
| pSVS112 | pEVS79 ΔrpoQ | This study |
| pTM214 | pVSV105 Ptrc-mCherry | 63 |
| pTM280 | pVSV105 PluxI-gfp + PtetA-mCherry | 11 |
| pTM319 | pEVS107 lacIqPtrc-rpoQ | This study |
| pXDC1 | pEVS79 ΔVFA1014 | This study |
| pXDC10 | pTM214 Ptrc-rpoQ ΔmCherry | This study |
| pXDC23 | pTM214 Ptrc-VF_A1016 ΔmCherry | This study |
| pXDC35 | pTM214 Ptrc-rpoS ΔmCherry | This study |
| pXDC36 | pTM214 Ptrc-rpoD ΔmCherry | This study |