Table 2.
Michaelis-Menten parameters of tested nanoparticles
Nanoparticle | TMB | ABTS | ||||
---|---|---|---|---|---|---|
KM (mmol/L) | Vmax (10−7Ms−1) | kcata (10−4s−1) | KM (mmol/L) | Vmax (10−7Ms−1) | kcata (10−4s−1) | |
Ncit | 0.24 | 1.70 | 0.81 | 0.73 | 1.45 | 0.69 |
NCMD | 0.30 | 1.25 | 0.60 | 0.81 | 1.15 | 0.55 |
Nhep | 0.22 | 2.40 | 1.14 | 0.96 | 0.52 | 0.25 |
Ngly | 0.55 | 0.96 | 0.46 | 0.20 | 2.97 | 1.41 |
NPLL | 0.69 | 0.46 | 0.22 | 0.19 | 4.50 | 2.14 |
NPEI | 0.71 | 0.42 | 0.20 | 0.12 | 6.10 | 2.90 |
Catalytic constant derived from kcat=Vmax/[E], where Vmax is the maximum reaction rate reached with increasing TMB or ABTS concentration, as shown in Figure 5; [E] is the nanoparticle iron concentration measured by inductively coupled plasma-optical emission spectroscopy (ICP-OES).