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. 2011 Oct 19;11(1):397–411. doi: 10.1021/pr200845n

Figure 5.

Figure 5

AtVPS2.2-GFP localizes to distinct region of the plasma membrane, in cytoplasmic vesicles separated for the trans-Golgi network (TGN) marker VHAa1 and in nuclei of root meristems. (A) Root tips of seedlings expressing AtVPS2.2-GFP, (B) root meristems of seedlings expressing AtVPS2.2-GFP and the TNG marker VHAa1-mRFP, (C) close up of the marked area in B demonstrating that AtVPS2.2-GFP localizes strongly to the transverse cell borders, to vesicles of different sizes in the cytoplasm and diffusely in the nucleus. (D) Root area at the upper end of the meristem with diffuse nuclear AtVPS2.2-GFP localization. (E) Same root area as in D but labeled with the fluorescent DNA stain, DAPI. (F) Overlay of D and E. (G) Venn diagram of the overlap between AtVPS2.2-GFP interactors and proteins that have been isolated from and annotated to the nucleus. The colored AGI codes of the proteins correspond to the overlapping sectors. The first authors of the publications used for comparison are also color coded.