Table 1.
Molecular assay | Primer | Sequence 5′–3′ and product size | Positiona |
---|---|---|---|
PCR envb | D498 forward | ATG GGT AAG TTT CTB GCC (18 bp) | 5202–5219 |
D500 reverse | TTA CAG GGA TGA CTB AGG (18 bp) | 6668–6651 | |
n-PCR envb | LUI 7 forward | CCG TCT CCA GYC CMT MCT GGA (21 bp) | 5547–5567 |
LUI 8 reverse | CCT CGT CTR TTY TGG GCW GCA (21 bp) | 6309–6289 | |
PCR LTRc | LTR-I.03 forward | GGC TTA GAG CCT CCC AGT GA (20 bp) | 57–76 |
LTR-I.02 reverse | CGC GGA ATA GGG CTA GCG CT (20 bp) | 864–845 | |
n-PCR LTRc | LTR-I.03 forward | GGC TTA GAG CCT CCC AGT GA (20 bp) | 57–76 |
LTR-I.04 reverse | GCC TAG GGA ATA AAG GGG CG (20 bp) | 822–803 |
Primer nucleotide position is provided as aligned with ATK (HTLV-1-infected cell line; GenBank Accession number J02029). bp, base pairs; n-PCR, nested polymerase chain reaction.
Adapted from Caterino-de-Araujo et al.11
Adapted from Laurentino et al.6
Primers were employed to detect HTLV-1 provirus DNA in peripheral blood leucocytes of HIV coinfected patients from the south (Londrina-LO) and the southeast (São Paulo-SP) regions of Brazil.