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. 2012 Jan;28(1):110–114. doi: 10.1089/aid.2011.0117

Table 1.

Primers Employed in Polymerase Chain Reaction Assays (env and LTR)

Molecular assay Primer Sequence 5′–3′ and product size Positiona
PCR envb D498 forward ATG GGT AAG TTT CTB GCC (18 bp) 5202–5219
  D500 reverse TTA CAG GGA TGA CTB AGG (18 bp) 6668–6651
n-PCR envb LUI 7 forward CCG TCT CCA GYC CMT MCT GGA (21 bp) 5547–5567
  LUI 8 reverse CCT CGT CTR TTY TGG GCW GCA (21 bp) 6309–6289
PCR LTRc LTR-I.03 forward GGC TTA GAG CCT CCC AGT GA (20 bp) 57–76
  LTR-I.02 reverse CGC GGA ATA GGG CTA GCG CT (20 bp) 864–845
n-PCR LTRc LTR-I.03 forward GGC TTA GAG CCT CCC AGT GA (20 bp) 57–76
  LTR-I.04 reverse GCC TAG GGA ATA AAG GGG CG (20 bp) 822–803
a

Primer nucleotide position is provided as aligned with ATK (HTLV-1-infected cell line; GenBank Accession number J02029). bp, base pairs; n-PCR, nested polymerase chain reaction.

b

Adapted from Caterino-de-Araujo et al.11

c

Adapted from Laurentino et al.6

Primers were employed to detect HTLV-1 provirus DNA in peripheral blood leucocytes of HIV coinfected patients from the south (Londrina-LO) and the southeast (São Paulo-SP) regions of Brazil.