Figure 1.

Upregulation of NEU3 in human prostate cancer. (a) Relative mRNA levels of NEU3 assessed by quantitative RT–PCR for 34 cancer and 35 non-cancerous samples. Box-and-whisker plots were used for statistical analysis. (b) Sialidase activity levels of cancer and non-cancerous samples assessed by fluorometric high-performance liquid chromatography with malononitrile. (c) Co-relationship between NEU3 level and prostate cancer grading, Gleason score. The data were statistically analyzed using the Kruskal–Wallis test. (d) Immunohistochemistry with anti-NEU3 monoclonal antibody for prostate cancer tissues with the high Gleason score and non-tumor prostate tissues