Figure 2.

Altered expression of differentiation- and apoptosis-related molecules by NEU3 in human prostate cancer cell lines. (a) NEU3 expression in mRNA and sialidase activity levels in LNCaP cells and PC-3 cells. The data given are mean values from five to seven experiments±S.D. P-values (^*P=0.015, ^**P=0.0003) were evaluated by the Student's t-test, and compared with PC-3 cells. (b) Apoptosis induction of PC-3 and LNCaP cells by treatment with sodium butyrate, and apoptosis suppression by NEU3 overexpression. Apoptosis was assessed by FACS analysis with AnnexinV. Results are representative of three independent experiments. (c) Measurement of mRNA levels of keratin 17 and IL24, and Bcl-2 in NEU3-silencing or -overexpressing cells. ^*P< 0.05, ^**P<0.01 compared with control siRNA or vector by the Student's t-test