Figure 5.

Acceleration of androgen-dependent cell growth by NEU3 in LNCaP cells. An increase in DHT-induced cell proliferation by NEU3 in LNCaP cells (a) and in AR silencing LNCaP cells (b), as determined by MTT assays. (c) Increased expression of NEU3 and EGR-1 (in RT–PCR, right panel) in AR silencing cells under conditions in which AR and PSA were reduced profoundly (in immunoblotting, left panel). Stimulated ERK phosphorylation seen by AR silencing (left panel). (d) Proposed model of a possible activation pathway of AR signaling regulated by NEU3 under androgen-deficient conditions