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. 2011 Jun 17;19(1):170–179. doi: 10.1038/cdd.2011.83

Figure 6.

Figure 6

Suppression of prostate cancer growth in vitro and in vivo by treatment with NEU3 siRNA. (a) Reduction in the number of LNCaP and PC-3 cells by treatment with NEU3-specific siRNA, as assessed by MTT assays. (b) Reduction of the tumor volumes of xenografts implanted PC-3 cells by NEU3 siRNA treatment with atelocollagen. Different doses of NEU3 siRNA (0, 250 or 500 pmol) were injected into three groups of athymic nude mice (n=10 for each condition) and the tumor diameters were measured and compared as described in Materials and Methods. The statistical analyses were performed using the Kruska–Wallis test. (c) HE staining (left panel) and immunohistological staining of tumor tissues from the mice with anti-Ki-67 and -NEU3 antibodies (two middle panels). Detection of apoptosis by TUNEL method in the tissues was carried out using an apoptosis detection kit (right panel)