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. 2011 Nov 2;7:1494–1498. doi: 10.3762/bjoc.7.173

Table 1.

Conversion (% relative peak-area by GC–MS of extracted reaction mixtures), after 24 h, of substrates 1, 3, 5, and, after 48 h, of substrates 7, 11, 13 and 15 with P450cam-RhFRed mutants in 48-well plate biotransformations.

graphic file with name Beilstein_J_Org_Chem-07-1494-i001.jpg
Compounds P450cam-RhFRed mutants
Substrate Products Substrate no. Y96A Y96F Y96F/V247A
Inline graphic
1 		Inline graphic
2 		1 >99 >99 >99
Inline graphic
3 		Inline graphic
4 		3 94 98 >99
Inline graphic
5 		Inline graphic
6 		5 64 50 >99
Inline graphic
7 		Inline graphic
10 		7a 12 (8a) — —
7b 48 (8b) 26 (8b) 73 (8b)a
7c 85 (10c)b 16 (8c)
34 (10c)		 7 (8c)
59 (10c)
31 (10c')
7d n.c.c n.c.c n.c.c
Inline graphic
11 		Inline graphic
12 		11a <2 (u)d — —
11b 12 (12b) <2 (u)d 9 (12b)
15 (12b')
11c <2 (u)e n.c.c 11 (12c)
11d 88 (12d)f 22 (12d)
69 (u)d 		18 (12d)
82 (u)d
Inline graphic
13 		Inline graphic
14 		13 n.c.c n.c.c 18 (14d)
6 (14d')
Inline graphic
15 		Inline graphic
16 		15 15 (16d)
9 (16d')		 11 (16d)
10 (16d')		 15 (16d)
75 (16d')

aOne other product was detected (8%). bOne other product was detected (10%). cn.c. = no conversion. du = unidentified product. eTwo unidentified products. fThree other minor products were detected (12%).