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Fluorescence spectra of Cd2+–ACAQ (10 µm) upon the titration of (a) D-Cys and (b) L-Cys in buffer solution (10 µM, HEPES, 1% ACN, pH 7.4 ) at 298 K with λex = 350 nm. Inset is the titration data point and the nonlinear least-squares fitting curve at 500 nm.
