Table 1.

ELISA anti-MUC1 and anti-T_helper antibody titers after four immunizations with various preparations

Immunization*	IgG total MUC1	IgG1 MUC1	IgG2a MUC1	IgG2b MUC1	IgG3 MUC1	IgM MUC1	IgG total Thelper
1 (no tumor induced)	31,900	10,600	10,000	15,500	3,900	100	2,100
1	30,200	16,000	6,600	10,700	3,900	50	3,000
2	12,900	10,400	4,100	4,500	700	100	1,000
3	1,300	0	100	900	0	0	50
4 + 5	300	0	0	200	0	0	1,000
5	0	0	200	0	0	50	50

Anti-MUC1 and anti-T_helper antibody titers are presented as median values for groups of 4–13 mice. ELISA plates were coated with BSA-MI-CTSAPDT(αGalNAc)RPAP conjugate for anti-MUC1 antibody titers or NeutrAvidin-biotin-T_helper for anti-T_helper antibody titers. Titers were determined by linear regression analysis, with plotting of dilution versus absorbance. Titers are defined as the highest dilution yielding an optical density of 0.1 or greater relative to normal control mouse sera.

*Liposomal preparations were used. MMT tumors were induced between the third and fourth immunization.