Skip to main content
. 2011 Dec 14;109(1):39-43. doi: 10.1073/pnas.1115778108

Fig. 3.

Fig. 3.

Spectroscopic comparison of [Re]-Br and [Re]-S355C-β2. The UV-vis absorption (brown) and emission spectra (λex = 355 nm) (brown) (50 μM) of [Re]-Br (MeCN solution) and the UV-vis absorption (blue) and emission (blue) (10 μM) spectra of [Re]-S355C-β2 (50 mM HEPES, 1 mM EDTA, pH 8.0) are shown. The absorption spectrum of S355C-β2 (gray) (50 mM HEPES, 1 mM EDTA, pH 8.0) is also included for reference. The simulated absorption spectrum (red) of [Re]-S355C-β2 by summing the absorption spectrum of twice the absorption spectrum of [Re]-Br and S355C-β2. The similarity of the actual (blue) and simulated (red) [Re]-S355C-β2 UV-vis absorption traces suggests that binding has no significant impact on the spectroscopic properties of [Re].