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. 2011 Dec 19;109(1):209–214. doi: 10.1073/pnas.1114647109

Fig. 1.

Fig. 1.

Cell cycle arrest caused by overexpression of Mph1. (A) Mph1 was overexpressed from pREP41-mph1+ in the wild-type cells or the indicated spindle checkpoint mutants. Gene expression is repressed in the media with thiamine (+thiamine) and derepressed in the media without thiamine (−thiamine). The plates were incubated at 32 °C for 3 d. (B) Mph1 kinase-dead (KD) mutant was overexpressed in the wild-type cells, mad2Δ, or mph1Δ from pREP41 as in A. (C) Centromere II's and microtubules were visualized by cen2-GFP and Atb2-mCherry, respectively. DNA was visualized by staining with Hoechst 33342. (Scale bars, 5 μm.) (D) Indexes of metaphase arrest: cen2 on the spindle (gray bars) and chromosome condensation (black bars) were determined in cells overexpressing Mph1-HA or Mph1KD-HA from pREP41, respectively. The error bars represent SEM of the three individual experiments.