Fig. 4.

Comparison of activity toward different α-methylcarboxylate esters for a small subset (89 transformants) of the combinatorial library. The transformants are ordered after increasing activity toward substrate 2. The highest activity for each substrate in this subset was normalized to 100. P. pastoris X33 supernatants were used as blank. The highest activity toward 1 found in this subset is an SV₁CAV₂ variant. For comparison, in this subset the initial rates for the best variants toward 1, 2, and 4 are 1.1, 22, and 32 mOD₄₁₀ min^-1, respectively.