Table 4.

Enantioselectivity of single and double mutants of CALA

Variant	E*†	Variant	E*†
SV1	4.0 ± 0.9	CV2	1.2 ± 0.1
SC	3.9 ± 0.8	AV2	1.3 ± 0.1 (R)
SA	1.9 ± 0.5	S	2.1 ± 0.3
SV2	1.4 ± 0.2	V1	1.1 ± 0.1 (R)
V1C	5.1 ± 0.8	C	1.3 ± 0
V1A	1.4 ± 0.3 (R)	A	1.5 ± 0.2 (R)
V1V2	2.0 ± 0.2	V2	1.1 ± 0 (R)
CA	1.3 ± 0.1

^*Enantioselectivity factor, calculated according to Table 2. At least three independent reactions were performed to validate the reported E values. Standard error of the mean is reported.

^†Unless otherwise noted the selectivity was in favor of the S enantiomer.