Fig. 1.
EpiBL perception does not induce PTI responses. (A) Oxidative burst triggered by flg22, elf18, or epiBL in Col-0 leaf discs. ROS production is presented as total photon counts during 40 min of treatment. Values are mean ± SE (n = 20). (B) Activation profile of MAPKs in response to a time-course treatment with 1 μM flg22 or epiBL in 2-wk-old Col-0 seedlings. Arrowheads indicate phosphorylated MPK3 and MPK6. Blots stained with colloidal brilliant blue (CBB) are presented to show equal loading. (C) Quantitative RT-PCR analysis of FRK1 and At2g17740 expression in 2-wk old Col-0 seedlings treated with 100 nM flg22 or epiBL for 0, 1, or 3 h. Transcript levels are normalized to the U-box gene and are presented as relative to the value at 0 h. Values are mean ± SD (n = 3). (D) Growth of Pto DC3000 in Col-0 leaves pretreated with water, 1 μM flg22, or epiBL for 24 h and then syringe-infiltrated with 105 cfu/mL of bacteria. Bacterial growth was determined 2 d after inoculation. Values are mean ± SE (n = 8; ***P < 0.001). Similar results were observed in at least two independent experiments.