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. 2011 Oct 27;109(1):303–308. doi: 10.1073/pnas.1109921108

Fig. 2.

Fig. 2.

Activation of BAK1 by PAMP perception does not modulate BR signaling. (A) BES1-GFP phosphorylation (detected as band shift) after the indicated times of seedling treatment with 1 μM epiBL and/or 1 μM flg22 by using anti-GFP antibodies. Blot stained with CBB is presented to show equal loading. (B) Phenotype of 2-wk-old Col-0, bri1-9, and bri1-301 Arabidopsis seedlings treated with buffer or different concentrations of flg22 or elf18 for 8 d. (Scale bar: 1 cm.) (C) Quantitative RT-PCR of CPD gene expression in 2-wk-old Col-0 seedlings grown with or without 10 nM flg22 for 1 wk and then treated with 1 μM epiBL for the indicated time. Transcript levels are normalized to the U-box gene and are presented as relative to mock treatment at time 0. Values are mean ± SD (n = 3; ***P < 0.001). Similar results were observed in at least three independent experiments.