Fig. 4.

Effects of ANP on inflammatory cascade activation induced by LTA or PepG. EC were pretreated with ANP (100 nM, 20 min), followed by treatment with LTA (100 ng/ml; A) or PepG (300 ng/ml; B) for 1, 3, or 6 h. Phosphorylation of p38, MYPT, and IκBα was determined by Western blot with corresponding antibodies. Degradation of IκBα was detected using pan IκBα antibodies. Equal protein loading was confirmed by probing of membranes with β-tubulin antibodies. Results are representative of five to seven independent experiments.