Fig. 5.

Effects of ANP on LTA- and PepG-induced EC permeability. A: ECs grown on microelectrodes to confluence were treated with LTA alone, PepG, or combination of LPA and PepG, followed by measurements of TER. Bar graphs represent polled TER data after 6 h of treatment. *P < 0.05, compared with LTA or PepG alone. B: lung EC monolayers were treated with ANP (100 ng/ml, 20 min, marked by first arrow). At the time point indicated by second arrow, cells were stimulated with a mixture of LTA (50 ng/ml) and PepG (100 ng/ml), and TER was monitored over the time. C: quantitative analysis of gap formation in control and treated HPAEC. Data are expressed as means ± SD of three independent experiments. *P < 0.05, compared with LTA/PepG. D: HPAEC were pretreated with vehicle or ANP (100 nM, 20 min), followed by stimulation with LTA and PepG for 6 h. Cytoskeletal remodeling was assessed by immunofluorescence staining for F-actin with Texas red phalloidin and VE-cadherin (merged images). Paracellular gaps are marked by arrows.