Fig. 7.

Effects of ANP on LTA- and PepG-induced lung vascular leak. C57BL/6J mice were treated with vehicle or a mixture of LTA (2.5 mg/kg) and PepG (2.5 mg/kg), with or without concurrent administration of ANP (2 μg/kg) for 24 h. Evans blue dye (30 ml/kg iv) was injected 2 h before termination of the experiment. A: lung vascular permeability was assessed by Evans blue accumulation in the lung tissue. B: the quantitative analysis of Evans blue-labeled albumin extravasation was performed by spectrophotometric analysis of Evans blue extracted from the lung tissue samples. Values are means ± SD; n = 4 per condition. *P < 0.05.