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. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: Virology. 2011 Nov 21;422(2):265–277. doi: 10.1016/j.virol.2011.10.024

Figure 5. ADAR1 induced mutations in the HIV-1 rev RNA.

Figure 5

293T cells were transfected with pNL4.3 and the ADAR1 plasmid DNAs. The RNA isolated from transfected cells were subjected to RT PCR amplification. Amplicons were cloned in pcDNA3.1 and 12–25 clones were picked (in the presence and absence of ADAR1) and sequenced. Mutational analysis of the nucleotide sequence of rev region of HIV-1 genome (Panel A). Four sequences are the representative of all clones with identical mutations. Comparison of amino acid sequences of HIV-1 rev region in the presence and absence of ADAR1 (Panel B).