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. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: Virology. 2011 Nov 21;422(2):265–277. doi: 10.1016/j.virol.2011.10.024

Figure 6. ADAR induced mutations in HIV-1 env gene.

Figure 6

Mutational analysis of the env region of HIV-1 genome in the presence and absence of ADAR1. 293T cells were transfected with pc DNA or ADAR1 constructs and RNA was isolated from infected cells. RT-PCR was done and c DNA of env was cloned in pc DNA vector. Each segment of the env gene was cloned individually (Segment 1 (6221 – 6873), Segment 2 (6872–7521), Segment 3 (7522–8187), and Segment 4 (8188–8803). 24 clones were randomly picked from each segment in presence and absence of ADAR1 and sequenced. In case of env 24 sequences were computed into 11 groups.