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. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: Virology. 2011 Nov 21;422(2):265–277. doi: 10.1016/j.virol.2011.10.024

Figure 9. A-to-G mutations in the proviral genome at position 6036, 8413 and 8438 affect the synthesis of viral protein.

Figure 9

A-to-G mutations were introduced at position 6036, 8413 and 8438 (single, double and triple) using site directed mutagenesis kit (Stratagene). 293T cells were transfected with 0.1µg mutant constructs and Renilla Luciferase plasmid. Viral protein synthesis was monitored by measuring HIV p24 in culture supernatant (Panel A) and intracellular HIV p24 production (Panel B). Viral gag RNA was quantitated in cells transfected with mutant constructs by Real-time RT-PCR (Panel C).