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. 2012 Jan 1;139(1):117–127. doi: 10.1242/dev.074450

Fig. 4.

Fig. 4.

PRC1 subunits are differentially required for repression of elB, noc, Doc2/3 and dac. (Top) ChIP profiles at the PRC1-bound genes elB, noc, Doc2/3 and dac, represented as in Fig. 3. (Below) Regulation of elB, noc, Doc2/3 and dac in wing imaginal discs with cell clones that lack individual PRC1 subunits. Row 1 shows wing discs from wild-type third instar larvae stained with antibodies against ElB, Noc, Doc2/3 and Dac proteins (magenta in each case) and co-stained with Hoechst (blue) to visualize nuclei. Rows 2 to 5 show wing discs with clones of cells lacking individual PRC1 subunits as indicated. Clones are marked by the absence of GFP and discs were analyzed 72 to 96 hours (rows 2 and 3) or 48 to 72 hours (rows 4 and 5) after clone induction. ElB, Noc, Doc2/3 and Dac proteins are all widely misexpressed in Ph or Psc-Su(z)2 mutant clones (white arrowheads). ElB and Noc are also misexpressed in Sce and Pc mutant clones (white arrowheads) but the Doc2/3 and dac genes remain stably repressed (open arrowheads).