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. 2011 Dec 1;138(23):5157–5166. doi: 10.1242/dev.069153

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© 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 4. — Selective ablation of αv integrin gene expression in astrocytes causes retinal angiogenesis pathologies. (A) Confirmation of Nestin-Cre-mediated deletion of αvflox gene in P14 retinas (r) but not matched tail snips (t). Genomic DNA was analyzed by PCR-based methods. Note that in the retinal sample there is a reduction in the ∼350 bp band owing to Cre-mediated recombination of the αvflox allele. (B) Detergent-soluble retinal lysates from P14 Nestin-Cre;αvflox/+ control or Nestin-Cre;αvflox/flox mice were immunoblotted with anti-αv antibodies. Note the diminished levels of αv integrin protein in mutant samples. (C) Images of retinas isolated from P14 Nestin-Cre;αvflox/+ control (left panel) or Nestin-Cre;αvflox/flox mutant mice (right panel) revealing intraretinal hemorrhage in mutant samples (arrows). (D,E) Retinas from P14 Nestin-Cre;αvflox/+ control (D) or Nestin-Cre;αvflox/flox mutant (E) mice were immunolabeled with anti-CD31 and anti-NG2 antibodies to visualize vascular endothelial cells and pericytes, respectively. Note the abnormal glomeruloid-like blood vessels comprising endothelial cells and pericytes in mutant samples (arrows in E). (F,G) Retinas from P14 Nestin-Cre;αvflox/+ control (F) or Nestin-Cre;αvflox/flox mutant (G) mice were immunolabeled with anti-CD31 and anti-GFAP antibodies to visualize vascular endothelial cells and astrocytes, respectively. Note that the abnormal vascular structures in mutant retinas (arrows in G) are associated with an apparently normal astrocyte network, although there is increased GFAP expression probably due to reactive astrogliosis resulting from intraretinal hemorrhage. Images in D-G are shown at 200× magnification.