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. 2011 Nov 3;1:27. doi: 10.1186/2045-9912-1-27

Figure 1.

Figure 1

Immunohistochemical staining of apoptotic and anti-apoptotic signals in the hippocampal Cornu Ammonis layer 1 (CA1) regions. A-C: Activated caspase-3 staining (green) revealed no apoptosis in isoflurane-exposed animals (A), whereas positive control from a mouse after 20-min of hypoxia exhibited activated caspase-3+ cells (B). C is higher magnification of area outlined in B. D-F: Bcl-xl immunostaining in the hippocampal CA1 regions (red) revealed no anti-apoptotic response in isoflurane-exposed animals (D), whereas positive control from a mouse after 20 min of hypoxia exhibited Bcl-xl+ cells (E). F is a higher magnification of area outlined in E. Cell nuclei are stained with DAPI (blue).