Figure 2.

Spinal blockade of PKCζ/PKMζ activity reduces the response of deep dorsal horn WDR neurons induced by intraplantar formalin. (a) Representative rate recordings of firing responses of WDR neurons to formalin following spinal pre-treatment with scrambled peptide or ZIP. (b) Time-course of WDR firing response to subcutaneous formalin (5%, 50 μl) injection into the hindpaw receptive field following spinal pre-treatment with scrambled peptide (10 μg, n = 8) or ZIP (10 μg, n = 8, ∗∗ p < 0.01 versus scrambled peptide). (c) Total neuronal activity during the 1st (0-10 min) and 2nd (10-70 min) phases of the formalin response with scrambled peptide or ZIP (2^nd phase: ∗∗∗ p < 0.001 versus scrambled peptide). Results are expressed as mean ± s.e.m.