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. 2011 Nov 5;7:86. doi: 10.1186/1744-8069-7-86

Figure 8.

Figure 8

Effect of spinal PKCζ/PKMζ inhibition on pain-related behaviors, PKCζ/PKMζ phosphorylation and Fos expression in spinal neurons following intraplantar CFA. Time-course of (a) mechanical and (b) thermal hypersensitivity in CFA rats following intrathecal administration of the scrambled peptide (10 μg, n = 8) or ZIP (10 μg, n = 8). Results are expressed as mean ± s.e.m. (∗ p < 0.05 versus scrambled peptide and o p < 0.05 versus the contralateral paw). (c, d) Representative photomicrographs of increased expression of p-PKCζ/p-PKMζ in superficial laminae of the ipsilateral lumbar dorsal horn following intraplantar CFA in rats pre-treated with intrathecal (c) scrambled peptide (10 μg, n = 5) or (d) ZIP (10 μg, n = 5). (e) Quantification of increased expression of p-PKCζ/p-PKMζ in the ipsilateral dorsal horn presented as a percentage of contralateral dorsal horn expression. (f, g) Representative photomicrographs of CFA-induced upregulation of Fos expression in superficial (I-II) and deep laminae (V-VI) of the ipsilateral lumbar dorsal horn in rats pre-treated with intrathecal (f) scrambled peptide (10 μg, n = 5) or (g) ZIP (10 μg, n = 5). (h) Quantification of number of Fos-positive cells in superficial and deep laminae of the lumbar (L4-L6) dorsal horn. All data presented as mean ± s.e.m. ∗ p < 0.05. Scale bars = 100 μm.