Table 2. Primers used in construction of PDR5 chimeras.
Primer | Sequence(5′-3′) |
#01 | CATTAATCCATTGGCTTACTT |
#02 | aactacgcgtTAGAGTAAAACCCG |
#03 | atatacgcgtCTGCAGCTGGACAG |
#04 | acatgcatgcACCGATGAGATAACCTAGAAAT |
#05 | tcttttaACcaagttGCAgaaagtgctgcaaacttag |
#06 | aatGAcaaGtagacccattgaaccaaCgtaaacgtag |
#07 | tctttatCtttctGtggtgttatgaccacacccag |
#08 | CatggtgaacaataaagAtgctaagtttgcagcac |
Bold: modified nucleotides.
Underlined: modified or newly generated endonuclease restriction sites.