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. 2012 Jan 6;7(1):e29761. doi: 10.1371/journal.pone.0029761

Figure 2. Fatty acid restores invadopodia following ACC1 inhibition.

Figure 2

(A) Invadopodia incidence in 3T3-Src cells treated with vehicle, TOFA (30 µM), or TOFA +16∶0 fatty acid for 24 hours. (B) Invadopodia incidence in 3T3-Src cells treated with vehicle, TOFA (30 µM), or TOFA +18∶1 fatty acid for 24 hours. (C) Invadopodia incidence in 3T3-Src cells transfected with 100 nM scrambled (Sc) siRNA or siRNA #1 against ACC1 for 48 hours and then supplemented with 18∶1 fatty acid for 24 hours. (D) ACC1 protein expression in 3T3-Src cells transfected with 100 nM scrambled or siRNA #1 against ACC1 and supplemented with 18∶1 fatty acid. (E) Cortactin localization to invadopodia in 3T3-Src cells treated with vehicle, TOFA (30 µM), or TOFA supplemented with 18∶1 fatty acid for 24 hours. Cortactin co-localization with F-actin was determined by immunofluorescence. Scale bar,1 µm. (F) 3T3-Src cells were treated with BSA or 18∶1 fatty acid for 24 hours and invadopodia incidence was determined. Scale bar, 25 µm *p≤0.05; **p≤0.01.