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. 2012 Jan 6;7(1):e29761. doi: 10.1371/journal.pone.0029761

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Scott et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Gelatin degradation of 3T3-Src cells seeded on glass cover slips coated with AlexaFluor-488 conjugated gelatin in the presence of vehicle, TOFA (30 µM), or TOFA with 18∶1 or 16∶0 fatty acid. Nuclei were visualized by staining with DAPI and actin was visualized with Alexafluor594-phalloidin. (B) Quantification of gelatin degradation by 3T3-Src cells transfected with scrambled (Sc) or siRNA #1 against ACC1 and supplemented with 18∶1 fatty acid for 24 hours. (C) Gelatin zymography of secreted MMP-2 in condition media from 3T3-Src cells treated with vehicle, TOFA (30 µM), or TOFA supplemented with 18∶1 fatty acid for 48 hours. Scale bar, 25 µm. *p≤0.05.