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. 2012 Jan 6;7(1):e29761. doi: 10.1371/journal.pone.0029761

Figure 8. ACC activity is important for invasive capabilities and lipid composition of cancer cells.

Figure 8

(A) Invasion through matrigel-coated invasion chambers was determined 3T3-Src cells transfected with 100 nM scrambled (Sc) or siRNA #2 against ACC1. Invasion was determined following staining with crystal violet. (B) Invasion through matrigel-coated invasion chambers was determined in 3T3-Src cells treated with vehicle, TOFA (30 µM), or TOFA + 36∶2 PC. Invasion was quantified as in A. (C) Invasion was determined in 3T3-Src cells treated with vehicle, TOFA (30 µM), or TOFA + 34∶1 PC. (D) MDA-MB-231 or (E) Snb-19 invasion through matrigel-coated chambers in the presence of vehicle or TOFA (30 µM). (F) The levels of 34∶1 PC in PC-3 cells treated vehicle or TOFA as determined by mass spectrometry. (G) Invasion of PC-3 cells through matrigel-coated invasion chambers in the presence of vehicle, TOFA (30 µM), or TOFA + 34∶1 PC. * p≤0.05; **p≤0.01; ***p≤0.001.