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. 2012 Jan 6;7(1):e29788. doi: 10.1371/journal.pone.0029788

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Daijun Ling.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Raw Ct data is imported as a temporary SAS dataset. It is recommended, but not required, to check data integrity and variable consistency using proper SAS procedures. SASqPCR sequentially calculates PCR efficiency, expression stability of candidate reference genes, optimal reference genes, normalized relative expression of target genes, and makes statistical testing. Results are automatically exported and saved. The exported results from each analytical step may serve as a reference for assigning input macro variables for the next step in the workflow. Users can also customize their analyses by arbitrarily excluding particular genes and/or samples. The interface of different computational components allows users to get optimal results. The “user-defined operation” refers to any user-developed programs to extend the analytical function of SASqPCR. Exp., expression; Ref., reference.