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. 2011 Oct 15;138(20):4499–4509. doi: 10.1242/dev.067454

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© 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 5. — Npnt knockdown causes cardiac jelly expansion and expanded expression of has2 and tbx2b. (A,B) Confocal sections from whole-mount F-actin-stained (red) control (A) and MO2-injected (B) Tg(kdrl:EGFP)^s843 zebrafish embryos at 52 hpf. The space between endocardium and myocardium was increased at the AV boundary (arrowheads) and throughout the chambers (red asterisks) in npnt morphants. (C-F) Sections of 52 hpf control and MO2-injected embryos after whole-mount in situ hybridization for has2 and tbx2b expression (brackets). Note that the expression of these genes is expanded in npnt morphants. (G-J) Sections (4 μm) of control, MO2-, has2 MO-, or has2 MO + MO2-injected zebrafish embryos at 52 hpf stained for hyaluronan (brown) and counterstained with Hematoxylin (blue). (K-N) Transmission electron microscopy of hearts (K,L) and endocardial cells (M,N) from control and MO2-injected embryos at 52 hpf. Arrowheads indicate junctional complexes. A, atrium; BC, blood cell; CJ, cardiac jelly; EC, endocardium; MC, myocardium; V, ventricle. Scale bars: 50 μm in A-F; 10 μm in G-J; 5 μm in K,L; 1 μm in M,N.