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. 2012 Jan 6;7(1):e30484. doi: 10.1371/journal.pone.0030484

Figure 4. The γ-secretase modulator photo-probe AR243 targets endogenous PSEN1 in human HEK293T cells.

Figure 4

Photo-affinity labeling studies were performed with membrane preparations from human HEK293T cells as described in Fig. 1. Western blotting of purified target proteins demonstrated labeling of endogenous PSEN1-NFT but not PSEN1-CTF by AR243. Co-incubation with an excess of parent compound BB25 (100 µM) caused displacement of the photo-probe, demonstrating specificity of the binding to PSEN1-NTF. Input represents 0.02% of the total membrane material.