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. Author manuscript; available in PMC: 2012 Oct 5.
Published in final edited form as: Cell Metab. 2011 Oct 5;14(4):555–566. doi: 10.1016/j.cmet.2011.09.004

Figure 6. Differential Response of Mitochondrial and Cytosolic NADH Level to Pyruvate and Lactate Supplementation.

Figure 6

(A–D) All experiments were performed in 293FT cells. Fluorescence with excitation at 485 nm was normalized to that of untreated cells. Shown are time courses of fluorescence response of FrexH-and cpYFP-expressing cells when 100 μM pyruvate (A) or 1 mM lactate (C) was added to the cell culture medium in the absence of glucose and time courses of fluorescence response of Frex-Mit- and cpYFP-Mit-expressing cells when 1 mM pyruvate (B) or 1 mM lactate (D) was added to the cell culture medium in the absence of glucose.

(E) Dose-dependent response of cytosolic NADH level to different concentration of lactate, as measured by Frex and FrexH fluorescence.

(F) AOA blocked the lactate-induced increase of mitochondrial NADH as measured by fluorescence of Frex-Mit 30 min after addition of lactate to the cell culture medium in the absence of glucose. Error bars represent SEM. See also Figure S5.