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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Neurochem. 2011 Nov 28;120(3):440–452. doi: 10.1111/j.1471-4159.2011.07576.x

Figure 4. Oligomeric αSyn reduces pCREB levels and coincidentally increases CaN activity in organotypic rat brain slices.

Figure 4

A. Representative Western blot (top) detecting pCREB in total protein extracts from organotypic brain slices treated with 0.75 µM of monomeric, oligomeric and fibrillar αSyn for the time length shown. The blot was stripped and re-probed for total CREB to control for sample gel loading. Densitometry band quantification (bottom) confirmed that pCREB levels were significantly reduced by aSyn oligomers but not monomers or fibrils. The graph shows the average from 3 independent experiments. *: p<0.05 vs. time 0 control (ANOVA).

B. CaN activity assay in homogenates from rat brain slices treated with 0.75 µM oligomeric αSyn for the times indicated. n=3 replicates per time point. *: p<0.05 vs. time 0 control (ANOVA).