Skip to main content
. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Immunol. 2011 Dec 19;188(2):774–780. doi: 10.4049/jimmunol.1101383

Fig. 2. MyD88 and UNC-93B mediate GBS- induced NO formation.

Fig. 2

A&B; BMDM lacking the indicated TLRs or TLR adaptors were stimulated (black) or not (gray) with GBS (107/ml). After 24 h, NO in the supernatant was determined. C. BMDM with a H417R mutation in UNC-93B were stimulated with GBS (107/ml), E. coli (107/ml) and LPS 100 ng/ml. After 24 h, NO in the supernatant was determined. D&E. BMDM were pre-treated with the indicated concentrations of cytochalasin D (µg/ml) or chloroquine (µM) for 1h before stimulation with GBS (106/ml). After 24h, NO in the supernatant was determined. F. BMDM were pre-treated with the indicated concentrations of cytochalasin D (µg/ml) for 1h before stimulation with GBS (106/ml) or LPS (100 ng/ml). After 24h, TNF and NO in the supernatant was determined. All the data is mean ± SD.