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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Immunol. 2011 Dec 19;188(2):774–780. doi: 10.4049/jimmunol.1101383

Fig. 5. iNOS is essential for the cytokine induction.

Fig. 5

A. BMDM from mice lacking gp91Phox (triangle), iNOS (circle), MYD88 (open square) and WT mice (filled square) were stimulated with GBS (105, 106, 107/ml). B. BMDM from WT mice (black) and iNOSKo mice (gray) were stimulated with live GBS for 2 hrs at MOI 10. Then, macrophages were washed extensively, and incubated for 8 hrs; Finally, TNF in the supernatants was measured by ELISA. Statistical significance: P-value *<0. 05 C. BMDM from WT mice (black) and iNOSKo mice (gray) were stimulated with GBS (106/ml), IL-6 & MIP-1 alpha in the supernatants was measured by ELISA. D. BMDM from WT mice were stimulated with GBS (106/ml) plus the indicated concentrations of sodium nitroprusside (SNP, µM) or SNP alone. E. WT-BMDM were pre-treated with chloroquine (µM) for 1h before stimulation with GBS (106/ml) or LPS (100ng/ml). After 24 h, TNF in the supernatants was measured by ELISA. Data is mean ± SD.