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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Proteome Res. 2011 Oct 25;11(1):85–94. doi: 10.1021/pr2009115

Figure 4. Discovery of the biosynthetic gene cluster for antibiotic S 213L in strain F-6133.

Figure 4

(a) The partial gene cluster determined by shotgun genome sequencing of strain F-6133. orf 12 NRPS contains peptides identified by LC-MS/MS and two PCR products from F-6133. Gene annotations were based on BLAST analysis. (b) SIC for m/z 799.39 detected in a culture supernatant from strain F-6133. The mass spectrum of the SIC peak is shown as an inset. Two masses separated by 52.9 Da (mass difference of Fe and 3 × H+) were shown, suggesting iron free and iron bound forms of the same species. (c) Structure of antibiotic S 213L. The labeled fragment ions were observed by tandem MS of the species at m/z 799.39.