Table 1.
LC-MS detection of the natural products produced by expressed NRPS/PKS gene clusters. The compound structures are shown in Figure 2.
| Strain NRRL# |
Gene Cluster Type |
Proposed Product | Predicted Structure |
Mass Detected (Da) |
Theoretical Mass (Da) |
Compound Name |
|---|---|---|---|---|---|---|
| F-6133 | PKS | bafilomycin | 1 | 815.4462 720.4095 |
815.4456 720.4085 |
bafilomycin B1 bafilomycin C1 |
| NRPS | siderophore | 2 | 798.387 | 798.3872 | antibiotic S213L | |
| F-6143 | NRPS | siderophore | Unknown | NDa | NAb | NA |
| F-6134 | PKS | filipin | 3 | ND | NA | NA |
| NRPS | actinomycin | 4 | 1270.6207 1240.6116, 1282.6216 1268.6085 |
1270.6234 1240.6128 1282.6234 1268.6077 |
actinomycin XOβ/ XOδ actinomycin D0 actinomycin Pip 1δ actinomycin X2 |
|
| F-6652 | NRPS | lipopeptide | unknown | ND | NA | NA |
| PKS-NRPS | tetramate macrolactam | 5 | ND | NA | NA | |
| F-6562 | NRPS | siderophore | 6 | 575.2558 | 575.2551 | foroxymithine |
| NRPS | enterobactin | 7 | 669.1475 | 669.1442 | enterobactin | |
| F-6556 | NRPS | fuscachelin | 8 | 1047.4368 | 1047.437 | fuscachelin B |
a
ND: not detected;
b
NA: not available