Table 1. List of the Redox-Sensitive Proteins Identified in This Studya.
| gene locus | protein function | number of peptides matchedb | coverage (%)b | methodc |
|---|---|---|---|---|
| Primary metabolism | ||||
| AT1G64190 | 6-phosphogluconate dehydrogenase | 9 | 23.0 | D/I |
| AT1G77120 | alcohol dehydrogenase 1 (ADH1) | 2 | 8.2 | B/B |
| AT3G48000 | aldehyde dehydrogenase 2B4 (ALDH2B4) | 7 | 15.0 | B/B |
| AT3G17940 | aldose 1-epimerase, putative | 5 | 16.0 | D/I |
| AT1G14810 | aspartate semialdehyde dehydrogenase | 4 | 15.0 | D/B |
| AT3G55440 | cytosolic triose phosphate isomerase | 10 | 42.0 | B/I |
| AT1G74030 | enolase 1 (ENO1) | 4 | 8.6 | D/I |
| AT2G36530 | enolase 2 (ENO2) | 3 | 9.7 | D/I |
| AT3G52930 | fructose-bisphosphate aldolase, putative | 11 | 49.0 | D/I |
| AT3G04120 | GAPDH C subunit 1 (GAPC-1) | 8 | 24.0 | D/I |
| AT1G13440 | GAPDH C subunit 2 (GAPC-2) | 4 | 13.0 | D/I |
| AT1G53240 | malate dehydrogenase (NAD), mitochondrial | 3 | 12.0 | D/I |
| AT5G43330 | malate dehydrogenase, cytosolic, putative | 2 | 5.4 | D/I |
| AT1G79550 | phosphoglycerate kinase (PGK) | 6 | 20.0 | B/I |
| AT5G50850 | pyruvate dehydrogenase | 2 | 6.1 | D/B |
| AT2G19940 | semialdehyde dehydrogenase family protein | 4 | 12.0 | D/I |
| AT1G01800 | short-chain dehydrogenase/reductase family protein | 7 | 35.0 | D/B |
| Antioxidant system | ||||
| AT3G11630 | 2-Cys peroxiredoxin (2-Cys PrxA) | 4 | 22.0 | B/I |
| AT1G07890 | ascorbate peroxidase (APX1) | 2 | 9.6 | B/I D/B |
| AT1G75270 | dehydroascorbate reductase 2 (DHAR2) | 2 | 9.9 | B/B |
| AT5G42980 | encodes a cytosolic thioredoxin | 4 | 40.0 | D/B |
| AT3G10920 | manganese superoxide dismutase (MSD1) | 2 | 9.5 | B/I |
| AT1G65980 | thioredoxin-dependent peroxidase 1 (TPX1) | 2 | 6.2 | D/B |
| Translational and post-translational control | ||||
| translation | ||||
| AT3G09200 | 60S acidic ribosomal protein P0 | 2 | 10.0 | D/I |
| AT1G07920 | elongation factor 1-alpha | 6 | 19.0 | D/I |
| AT5G60390 | elongation factor 1-alpha | 6 | 19.0 | D/I |
| AT1G07940 | elongation factor 1-alpha | 6 | 19.0 | D/I |
| AT1G07930 | elongation factor 1-alpha | 6 | 19.0 | D/I |
| AT5G19510 | elongation factor 1B alpha-subunit 2 (eEF1Balpha2) | 4 | 26.0 | D/B |
| AT4G11120 | translation elongation factor Ts (EF-Ts) | 3 | 7.8 | D/B |
| protein folding | ||||
| AT2G04030 | a chloroplast-targeted 90-kDa heat shock protein | 3 | 4.7 | B/B |
| AT1G21750 | protein disulfide isomerase-like protein (AtPDIL1-1) | 14 | 29.0 | B/B |
| AT1G77510 | protein disulfide isomerase-like protein (AtPDIL1-2) | 10 | 30.0 | B/B |
| AT3G54960 | protein disulfide isomerase-like protein (AtPDIL1-3) | 10 | 21.0 | B/B |
| AT5G60640 | protein disulfide isomerase-like protein (AtPDIL1-4) | 10 | 17.0 | B/I B/B |
| AT3G16110 | protein disulfide isomerase-like protein (AtPDIL1-6) | 5 | 9.7 | D/I B/B |
| AT2G47470 | protein disulfide isomerase-like protein (AtPDIL2-1) | 5 | 16.0 | D/B B/B |
| AT1G56340 | calreticulin1 (CRT1) | 2 | 4.5 | B/I |
| AT2G28000 | chaperonin-60 alpha (CPN60A) | 15 | 30.0 | D/I B/I B/B |
| AT3G56070 | cytosolic cyclophilin (CYP2) | 4 | 24.0 | B/I |
| AT1G60420 | DC1 domain-containing protein/PDI-like protein | 10 | 19.0 | D/I |
| AT3G12580 | heat shock protein 70(HSP70) | 15 | 29.0 | D/I |
| AT5G28540 | luminal-binding protein 1(BIP1) | 18 | 32.0 | D/I |
| AT4G37910 | mitochondrial heat shock protein 70-1 (MTHSC70-1) | 24 | 44.0 | D/I B/I |
| AT4G26110 | nucleosome assembly protein 1;1 (NAP1;1) | 3 | 8.6 | B/I |
| AT3G62030 | rotamase/CYP 4 | 2 | 8.1 | B/I |
| protein degradation | ||||
| AT5G35590 | 20S proteasome alpha subunit A1 (PAA1) | 2 | 9.8 | D/B |
| AT1G16470 | 20S proteasome alpha subunit B1 (PAB1) | 5 | 20.0 | B/I |
| AT2G27020 | 20S proteasome alpha subunit G1 (PAG1) | 2 | 8.4 | D/B |
| AT3G26340 | 20S proteasome beta subunit E, putative | 2 | 9.5 | B/I |
| AT5G42790 | 26S proteasome alpha subunit F1 (PAF1) | 6 | 25.0 | D/I B/I |
| AT4G12060 | double Clp-N motif protein | 2 | 9.1 | B/I |
| AT4G20850 | Tripeptidyl Peptidase II | 10 | 7.6 | B/B |
| AT2G17190 | ubiquitin family protein | 4 | 9.3 | B/I |
| post-translational modifications | ||||
| AT1G50370 | phosphoprotein phosphatase | 2 | 6.6 | D/I |
| AT5G53140 | protein phosphatase 2C, putative (PP2C) | 4 | 9.0 | B/I |
| AT1G71860 | protein tyrosine phosphatase 1(PTP1) | 4 | 13.0 | D/I |
| Cytoskeleton | ||||
| AT5G09810 | actin 7 (ACT7) | 10 | 31.0 | D/I B/I |
| AT4G14960 | tubulin alpha-6 chain (TUA6) | 11 | 36.0 | D/I |
| AT5G62690 | tubulin beta-2 (TUB2) | 5 | 11.0 | D/I |
| Others | ||||
| AT5G10450 | 14-3-3 lambda | 4 | 21.0 | B/I |
| AT4G37000 | accelerated cell death 2 (ACD2) | 4 | 14.0 | D/I |
| AT1G12910 | anthocyanin1 (AtAN11) | 3 | 8.4 | B/I |
| AT5G10920 | argininosuccinate lyase (AtArgH) | 5 | 8.5 | D/I |
| AT4G24830 | arginosuccinate synthase family protein | 11 | 25.0 | D/I |
| AT5G18400 | AtCIAPIN1 | 3 | 11.0 | D/B |
| AT1G78900 | catalytic subunit A of the vacuolar ATP synthase | 19 | 35.0 | D/I B/I |
| AT1G13870 | deformed roots and leaves 1 (DRL1) | 2 | 7.9 | D/B |
| AT3G25530 | gamma-hydroxybutyrate dehydrogenase | 2 | 7.6 | D/I |
| AT4G34540 | isoflavone reductase family protein | 2 | 6.9 | D/I |
| AT3G07720 | kelch repeat-containing protein | 2 | 6.7 | D/I |
| AT5G48480 | lactoylglutathione lyase | 4 | 27.0 | B/I |
| AT3G44300 | nitrilase 2 (NIT2) | 5 | 15.0 | D/B B/B |
| AT4G08790 | nitrilase, putative | 2 | 6.8 | D/I |
| AT1G63000 | nucleotide-rhamnose synthase/epimerase-reductase | 4 | 14.0 | D/I D/B |
| AT3G62530 | PBS lyase, HEAT-like repeat-containing protein | 3 | 13.0 | B/I |
| AT3G07090 | PPPDE putative thiol peptidase family protein | 3 | 13.0 | D/I |
| AT4G14000 | Putative methyltransferase family protein | 4 | 19.0 | B/I |
| AT2G38230 | pyridoxine biosynthesis 1.1 (ATPDX1.1) | 5 | 18.0 | D/I |
| AT5G01410 | pyridoxine biosynthesis 1.3 (ATPDX1.3) | 3 | 11.0 | B/I |
| AT1G07750 | RmlC-like cupins superfamily protein | 4 | 14.0 | D/B B/B |
| AT2G41530 | S-formylglutathione hydrolase | 2 | 7.4 | D/I |
| AT1G09760 | U2 small nuclear ribonucleoprotein A | 4 | 20.0 | D/I B/I |
| AT4G21580 | zinc-binding dehydrogenase family protein | 6 | 20.0 | D/I |
a
More detailed information on protein identification and properties are listed in Tables S1 and S2 (Supporting Information).
b
For proteins identified from multiple spots, derived from the spot yielding most numbers of uniquely matched peptides.
c
The method by which the protein was identified: D/I, direct IAF labeling. B/I, blocking-IAF labeling. D/B, direct BIAM tagging. B/B, blocking-BIAM tagging.