Table 2.
Peptide intensity ratios of cytochrome c, bovine serum albumin and myoglobin obtained with NHS-trypsin run-to-run and batch-to-batch digestion after RPLC-ESI-MS/MS analysis in triplicates.
| Sequence | Charge | m/z | Ratio of intensity | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Run2/Run1 | Run3/Run2 | Run3/Run1 | Batch2/Batch1 | Batch3/Batch2 | Batch3/Batch1 | ||||
| Cytochrome c | GEREDLIAYLKK | 3 | 478.9374 | 0.92 | 0.91 | 0.84 | 0.94 | 0.81 | 0.76 |
| TGQAPGFSYTDANK | 2 | 728.8392 | 1.04 | 0.86 | 0.90 | 1.17 | 0.91 | 1.06 | |
| TGPNLHGLFGR | 2 | 584.8159 | 1.09 | 0.86 | 0.94 | 1.24 | 0.84 | 1.04 | |
| Bovine serum albumin | SLHTLFGDELcK | 3 | 473.9035 | 0.92 | 0.83 | 0.77 | 0.91 | 0.68 | 0.62 |
| KVPQVSTPTLVEVSR | 3 | 547.3178 | 0.90 | 0.83 | 0.75 | 0.95 | 0.70 | 0.67 | |
| YIcDNQDTISSK | 2 | 722.3263 | 0.99 | 0.78 | 0.77 | 1.16 | 0.57 | 0.66 | |
| Myoglobin | ALELFRNDIAAK | 2 | 680.8832 | 0.71 | 0.99 | 0.70 | 1.06 | 0.84 | 0.90 |
| YKELGFQG | 2 | 471.2407 | 0.81 | 0.93 | 0.75 | 1.02 | 0.98 | 1.00 | |
| HLKTEAEMK | 2 | 543.7848 | 0.98 | 0.71 | 0.70 | 1.18 | 0.80 | 0.94 | |