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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Immunol. 2011 Dec 14;188(2):714–723. doi: 10.4049/jimmunol.1101404

FIGURE 2.

FIGURE 2

Intravaginal vaccination with HPV PsVs-Gag induces both cell mediated and humoral immune responses to SIV Gag. (A) Western blot showing the expression of the SIV Gag poly-protein (p55) in 293TT-cells following transduction with HPV16–SIV Gag constructs (lanes 2 & 3). Recombinant Gag p27 was loaded as a positive control in lane 1, degradation products of the recombinant protein are seen below the p27 band. Lysates from non-transduced cells were run as a negative control (lane 4). (B) IFN-γ responses measured by ELISpot after stimulation with SIV Gag peptides. (C) SIV Gag serum titers of IgG measured post HPV vaccination. (D) SIV Gag-specific IgA as a function of total IgA in vaginal secretions.