Fig. 7.

Peptidyl prolyl isomerase (Pin1)was dispensable for D,L-sulforaphane (SFN)-induced apoptosis. A: Immunoblotting for Pin1 using lysates from MDA-MB-231 and MCF-7 cells treated for 16-or 24 -hour with dimethyl sulfoxide (DMSO)or the indicated concentrations of SFN. Numbers above the bands represent densitometric quantitation of change in protein level relative to corresponding DMSO-treated control at each time point. B: Immunoblotting for Pin1 using lysates for MCF-7 cells stably transfected with empty vector or vector encoding for myc-Pin1 and treated for 24 hours with DMSO or the indicated concentrations of SFN. C: Histone-associated DNA fragment release into the cytosol, and D: cell viability in MCF-7 cells stably transfected with empty vector or vector encoding for myc-Pin1 and treated for 24 hours with DMSO or the indicated concentrations of SFN. Results are expressed relative to DMSO-treated cells transfected with empty vector (mean ± SD, n = 3). Significantly different (P<0.05) compared with ^arespective DMSO-treated control, and ^bbetween SFN-treated empty vector transfected cells and SFN-treated myc-Pin1 overexpressing cells by one-way ANOVA followed by Bonferroni’s test. Each experiment was done at least twice, and representative data from a single experiment are shown.