Table 2.

Fluorometric enzyme assay determination of caspase-3 activity in mice treated with anti-Fas antibody vs untreated controls.

	Caspase-3 activity (AFU/min/μg protein)
	Control (N=6)	Treated (N=10)
Blood**	0.11 ± 0.089 (0 – 0.21)	35 ± 34* (0.53 – 82)
Fat	0.76 ± 0.38 (0.29 – 1.3)	20.3 ± 21.8* (0.21 – 72.8)
Heart†	1.7 ± 0.7 (1.1 – 2.9)	18.9 ± 13.6* (2.5 – 41.2)
Kidney	1.44 ± 1.11 (0.11 – 3.37)	13.3 ± 12.4* (0.6 – 37.7)
Liver	8.6 ± 5.8 (2.2 – 18)	481 ± 205* (32 – 724)
Lung	2.2 ± 0.57 (1.4 – 3.0)	43 ± 23* (5.7 – 76)
Muscle	0.27 ± 0.29 (0.00 – 0.80)	7.91 ± 8.47* (0.42 – 28.7)
Spleen	28.2 ± 11.6 (17.1 – 49.1)	65.6 ± 38.5* (29.3 – 166)

Data expressed as mean ± standard deviation (range).

AFU = Arbitrary fluorescence units

^*p<0.05

^**N=8 in the Treated group for this organ.

^†N=5 in the control group. Including a single high outlier (N=6), the mean and standard deviation for this group was 3.4 ± 4.4 (range 1.1 – 12). The difference remained statistically significant including the high outlier in the analysis.